rabbit anti lhcgr  (Bioss)

Journal: Reproductive biology and endocrinology : RB&E

Article Title: Enhancing endometrial receptivity: the roles of human chorionic gonadotropin in autophagy and apoptosis regulation in endometrial stromal cells.

doi: 10.1186/s12958-024-01205-x

Figure Lengend Snippet: Fig. 7 hCG intervention changes the level of autophagy in endometrial cells. A The mRNA expression levels of Beclin1, LC3-II and P62 in ESCs were stimulated by hCG for 72 h. B The protein expression levels of Beclin1, LC3 and P62 in ESCs were stimulated by hCG for 72 h. C Representative images of LC3-II in ESCs treated with hCG for 72 h. Scale: 50 μm. D The mRNA expression levels of Beclin1, LC3-II and P62 in ESCs after the knockdown of LHCGR. E The protein expression levels of Beclin1, LC3 and P62 in ESCs were stimulated by hCG and knockdown of LHCGR. Data were presented as mean ± SD (n = 3): *P < 0.05, **P < 0.01

Article Snippet: They were then incubated for 30 min with 3% bovine serum albumin (BSA) to block nonspecific antibody binding and overnight at 4 °C with rabbit anti-LHCGR antibody (1:200 dilution; Proteintech) or rabbit anti-LC3 antibody (1:300 dilution; Proteintech).

Techniques: Expressing, Knockdown

Journal: Biology of reproduction

Article Title: Phoenixin-14 as a novel direct regulator of porcine luteal cell functions.

doi: 10.1093/biolre/ioad138

Figure Lengend Snippet: Figure 4. In vitro effect of phoenixin-14 (PNX-14) at doses 1–1000 nM on prostaglandin E2 (PGE2) (A) and F2α (PGF2α) (B) secretion as well as mRNA and protein expression of PTGER2 (C, E) and PTGFR (D, F) in porcine luteal cells. The protein abundance was analyzed by western blot method. Results are shown as representative immunoblots and a bar graph with densitometry measurement of relative target protein content normalized with actin protein. The data are presented as the mean ± SEM (n = 6). Statistical analysis was carried out using one-way ANOVA, followed by Tukey’s test. Different capital letters indicate significant differences at P <0.05.

Article Snippet: Supplier Phoenixin-14 Rabbit 1:200 (WB) G-079-01 Phoenix Pharmaceuticals Phoenixin-14 Rabbit 1:100 (IHC) H-079-01 Phoenix Pharmaceuticals GPR173 Rabbit 1:500 (WB) 1:100 (IHC) PA5-50976 Thermo Fisher Scientific STAR Rabbit 1:500 ab233427 Abcam CYP11A1 Rabbit 1:1000 ab175408 Abcam HSD3B Mouse 1:1000 ab75710 Abcam CYP19A1 Rabbit 1:200 PA1-21398 Thermo Fisher Scientific LHCGR Rabbit 1:200 19968-1-AP ProteinTech PTGER2 Rabbit 1:200 PA5-91872 Thermo Fisher Scientific PTGFR Rabbit 1:500 PA5-70674 Thermo Fisher Scientific pERK1/2 Rabbit 1:1000 #9101S Cell Signaling Technology ERK1/2 Rabbit 1:0000 #9102S Cell Signaling Technology pPKA Rabbit 1:500 ab5815 Abcam PKA Rabbit 1:500 ab187515 Abcam pAKT Rabbit 1:500 #9271S Cell Signaling Technology AKT Rabbit 1:500 #9272S Cell Signaling Technology pAMPK Rabbit 1:500 PA5-17831 Thermo Fisher Scientific AMPK Rabbit 1:500 PA5-17398 Thermo Fisher Scientific pPKC Rabbit 1:700 ab180848 Abcam PKC Rabbit 1:700 ab32376 Abcam Actin Mouse 1:1000 A5316 Sigma-Aldrich Anti-rabbit Goat 1:1000 #7074 Cell Signaling Technology Anti-mouse Horse 1:1000 #7076 Cell Signaling Technology Table 2.

Techniques: In Vitro, Expressing, Quantitative Proteomics, Western Blot